assays mouse corticosterone elisa kit elabscience  (Elabscience Biotechnology)

Journal: Clinical Traditional Medicine and Pharmacology

Article Title: Adaptogenic action of diosgenin againsts chronic unpredictable mild stress-induced neuroimmune dysfunction of HPA axis reverses psychiatric behavior in mice

doi: 10.1016/j.ctmp.2024.200148

Figure Lengend Snippet: Fig. 5. A-C: Diosgenin ameliorates CUMS-induced alterations in blood glucose (A) and serum corticosterone concentration (B) and reduced adrenal hypertrophy (C) in mice. After CUMS exposure and treatment with DG once per day for 21 days, serum glucose level was measured on day 21 following blood extraction ( ≤ 50 μL) from the tail vein prior to euthanasia. Corticosterone level was assayed with an ELISA kit with centrifuged serum after blood was collected from the left ventricle of the heart. Each adrenal gland was weighed using a sensitive weighing balance to assess for adrenal hypertrophy. Bars represent the mean ± SEM of 9 animals per group. ∗∗∗ P < 0.001 compared to VEH normal group; # P < 0.05, ## P < 0.01 compared to CUMS model (one-way ANOVA followed by Bonferroni post-hoc test). VEH = Vehicle, CUMS = Chronic unpredictable mild stress, DG = Diosgenin, FLU = Fluoxetine.

Article Snippet: We then assayed for corticosterone release with Elabscience orticosterone ELISA kit (Cat No. E-EL-0161, Elabscience Biotechnology o. Ltd , Wuhan, China), following the manufacturer’s manual.

Techniques: Concentration Assay, Extraction, Enzyme-linked Immunosorbent Assay

Journal: Clinical Traditional Medicine and Pharmacology

Article Title: Adaptogenic action of diosgenin againsts chronic unpredictable mild stress-induced neuroimmune dysfunction of HPA axis reverses psychiatric behavior in mice

doi: 10.1016/j.ctmp.2024.200148

Figure Lengend Snippet: Fig. 7. A-B: Diosgenin reduces CUMS-mediated release of tumor necrosis factor (TNF- 𝛼) (A) and interleukin-6 (IL-6) (B) in mice prefrontal cortex and hippocampus. Inflammatory cytokines such as TNF- 𝛼and IL-6 were measured with ELISA kits protocol using the supernatants of the mice brain regions (prefrontal cortex, striatum, and hippocampus) exposed to CUMS and treated with DG once per day for 21 days. Bars represent the mean ± SEM of 5 animals per group. ∗∗∗ P < 0.001 compared to VEH normal group; ## P < 0.01, ### P < 0.001 compared to CUMS model. VEH = Vehicle, CUMS = Chronic unpredictable mild stress, DG = Diosgenin, FLU = Fluoxetine.

Article Snippet: We then assayed for corticosterone release with Elabscience orticosterone ELISA kit (Cat No. E-EL-0161, Elabscience Biotechnology o. Ltd , Wuhan, China), following the manufacturer’s manual.

Techniques: Enzyme-linked Immunosorbent Assay